Glucose-6-Phosphate Dehydrogenase Protein (G6PD) (AA 1-491)
Quick Overview for Glucose-6-Phosphate Dehydrogenase Protein (G6PD) (AA 1-491) (ABIN666886)
抗原
See all Glucose-6-Phosphate Dehydrogenase (G6PD) 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 1-491
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序列
- MAVTQTAQAC DLVIFGAKGD LARRKLLPSL YQLEKAGQLN PDTRIIGVGR ADWDKAAYTK VVREALETFM KETIDEGLWD TLSARLDFCN LDVNDTAAFS RLGAMLDQKN RITINYFAMP PSTFGAICKG LGEAKLNAKP ARVVMEKPLG TSLATSQEIN DQVGEYFEEC QVYRIDHYLG KETVLNLLAL RFANSLFVNN WDNRTIDHVE ITVAEEVGIE GRWGYFDKAG QMRDMIQNHL LQILCMIAMS PPSDLSADSI RDEKVKVLKS LRRIDRSNVR EKTVRGQYTA GFAQGKKVPG YLEEEGANKS SNTETFVAIR VDIDNWRWAG VPFYLRTGKR LPTKCSEVVV YFKTPELNLF KESWQDLPQN KLTIRLQPDE GVDIQVLNKV PGLDHKHNLQ ITKLDLSYSE TFNQTHLADA YERLLLETMR GIQALFVRRD EVEEAWKWVD SITEAWAMDN DAPKPYQAGT WGPVASVAMI TRDGRSWNEF E
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产品特性
- G6PD,1-491aa, E.coli, Recombinant, E.coli
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纯化方法
- > 95% by SDS-PAGE
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Biological Activity Comment
- Specific activity is > 50unit/mg obtained by measuring the increase of beta-NADPH in absorbance at 340 nm resulting from the reduction of beta-NADP. One unit oxidizes 1.0 umole D-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of beta-NADP at pH 7.4 at 25C.
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Glucose-6-Phosphate Dehydrogenase (G6PD) (AA 1-515) protein (GST tag)
G6PD 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Glucose-6-Phosphate Dehydrogenase (G6PD) (AA 1-491) protein (His-IF2DI Tag)G6PD 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Glucose-6-Phosphate Dehydrogenase (G6PD) (AA 1-515) (Active) protein (His tag)G6PD 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 95 % SDS, EAA Active
Glucose-6-Phosphate Dehydrogenase (G6PD) (AA 1-491) (Active) proteinG6PD 宿主: E. coli 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % pure SDS Active
Glucose-6-Phosphate Dehydrogenase (G6PD) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)G6PD 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Phosphate-Buffered Saline ( pH 7.4) containing 10 % glycerol, 0.1 mM PMSF, 2 mM EDTA, 0.5 mM DTT
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- Glucose-6-Phosphate Dehydrogenase (G6PD)
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别名
- G6PD
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背景
- Glucose-6-phosphate dehydrogenase (G6PD) is the rate-limiting enzyme of the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells by maintaining the level of NADPH. G6PD converts glucose-6-phosphate into 6-phosphoglucono-delta-lactone and simultaneously produce NADPH. The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. G6PD deficiency cause acute hemolytic anemia. Recombinant G6PD protein was expressed in E. coli and purified by conventional chromatography techniques.
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分子量
- 55.7 kDa (491aa) confirmed by MALDI-TOF
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NCBI登录号
- NP_416366
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途径
- Regulation of Systemic Arterial Blood Pressure by Hormones
抗原
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